Scientific Advisory Committee

• B.A., Yale University
• M.D., Harvard Medical School
• Ph.D., University of Rochester

Research Interests

• Chondrocyte differentiation
• Growth plate chondrocytes
• Signaling pathways in chondrocytes
• Lead toxicity in chondrocytes

Representative Publications

• Wang Q, Wei X, Zhu T, Zhang M, Shen R, Xing L, O’Keefe RJ, Chen D. 2007. Bone Morphogenetic Protein 2 Activates Smad6 Gene Transcription through Bone-specific Transcription Factor Runx2. J Biol Chem. Apr 6;282(14):10742-8.
• Chen M, Lichtler AC, Sheu TJ, Xie C, Zhang X, O'Keefe RJ, Chen D. 2007. Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase. Genesis. Jan;45(1):44-50.
• Schwarz EM, Looney RJ, Drissi MH, O'Keefe RJ, Boyce BF, Xing L, Ritchlin CT. 2006. Autoimmunity and bone. Ann N Y Acad Sci. Apr;1068:275-83.
• Robertson G, Xie C, Chen D, Awad H, Schwarz EM, O’Keefe RJ, Guldberg RE, Zhang X. 2006. Alteration of femoral bone morphology and density in COX-2-/- mice. Bone. May 25;
• Li TF, Chen D, Wu Q, Chen M, Sheu TJ, Schwarz EM, Drissi H, Zuscik M, O’Keefe RJ. 2006. TGF-beta stimulates cyclin D1 expression through activation of beta -catenin signaling in chondrocytes. J Biol Chem. 2006 May 10
• Shen R, Wang X, Drissi H, Liu F, O’Keefe RJ, Chen D. 2006. Cyclin d1-cdk4 induce runx2 ubiquitination and degradation. J Biol Chem. Jun 16;281(24):16347-16353.
• Maloney MD, Goater JJ, Parsons R, Ito H, O’Keefe RJ, Rubery PT, Drissi MH, Schwarz EM. 2006. Safety and efficacy of ultraviolet-a light-activated gene transduction for gene therapy of articular cartilage defects. J Bone Joint Surg Am. Apr;88(4):753-761.
• Dong YF, Soung do Y, Schwarz EM, O’Keefe RJ, Drissi H. 2006. Wnt induction of chondrocyte hypertrophy through the Runx2 transcription factor. J Cell Physiol. Jul;208(1):77-86.
• Nishio Y, Dong Y, Paris M, O’Keefe RJ, Schwarz EM, Drissi H. 2006. Runx2-mediated regulation of the zinc finger Osterix/Sp7 gene. Gene. May 10;372:62-70.
• Li TF, Darowish M, Zuscik MJ, Chen D, Schwarz EM, Rosier RN, Drissi H, O’Keefe RJ. 2006. Smad3-deficient chondrocytes have enhanced BMP signaling and accelerated differentiation. J Bone Miner Res. Jan;21(1):4-16.
• Looney RJ, Schwarz EM, Boyd A, O’Keefe RJ. 2006. Periprosthetic osteolysis: an immunologist's update. Curr Opin Rheumatol. Jan;18(1):80-87. Review.
• Wang Y, Belflower RM, Dong YF, Schwarz EM, O’Keefe RJ, Drissi H. 2005. Runx1/AML1/Cbfa2 Mediates Onset of Mesenchymal Cell Differentiation Toward Chondrogenesis. J Bone Miner Res. 2005 Sep;20(9):1624-1636
• Koefoed M, Ito H, Gromov K, Reynolds DG, Awad HA, Rubery PT, Ulrich-Vinther M, Soballe K, Guldberg RE, Lin AS, O’Keefe RJ, Zhang X, Schwarz EM. 2005. Biological effects of rAAV-caAlk2 coating on structural allograft healing. Mol Ther. Aug;12(2):212-218.
• Dong Y, Drissi H, Chen M, Chen D, Zuscik MJ, Schwarz EM, O’Keefe RJ. 2005. Wnt-mediated regulation of chondrocyte maturation: modulation by TGF-beta. J Cell Biochem. Aug 1;95(5):1057-1068.
• Soloviev A, Schwarz EM, Darowish M, O’Keefe RJ. 2005. Sphingomyelinase mediates macrophage activation by titanium particles independent of phagocytosis: A role for free radicals, NFkappaB, and TNFalpha. J Orthop Res. Jun 8; [Epub ahead of print]
• Wei X, Zhang X, Zuscik MJ, Drissi MH, Schwarz EM, O'Keefe RJ. 2005. Fibroblasts express RANKL and support osteoclastogenesis in a COX-2-dependent manner after stimulation with titanium particles. J Bone Miner Res. Jul;20(7):1136-1148
• Carmouche JJ, Puzas JE, Zhang X, Tiyapatanaputi P, Cory-Slechta DA, Gelein R, Zuscik M, Rosier RN, Boyce BF, O'Keefe RJ, Schwarz EM. Lead exposure inhibits fracture healing and is associated with increased chondrogenesis, delay in cartilage mineralization, and a decrease in osteoprogenitor frequency. Environ Health Perspect. Jun;113(6):749-755.
• Gordon AH, O'Keefe RJ, Schwarz EM, Rosier RN, Puzas JE. 2005. Nuclear factor-kappaB-dependent mechanisms in breast cancer cells regulate tumor burden and osteolysis in bone. Cancer Res. 2005 Apr 15;65(8):3209-3217.
• Li TF, Zuscik MJ, Ionescu AM, Zhang X, Rosier RN, Schwarz EM, Drissi H, and O’Keefe RJ. 2004. PGE2 inhibits chondrocyte differentiation through PKA and PKC signaling. Exp Cell Res. Oct 15;300(1):159-169
• Ferguson CM, Schwarz EM, Puzas JE, Zuscik MJ, Drissi H, and O’Keefe RJ. 2004. Transforming growth factor-beta1 induced alteration of skeletal morphogenesis in vivo. J Orthop Res. Jul;22(4):687-696.
• Zuscik MJ, Baden JF, Wu Q, Sheu TJ, Schwarz EM, Drissi H, O’Keefe RJ, Puzas JE, and Rosier RN. 2004. 5-azacytidine alters TGF-beta and BMP signaling and induces maturation in articular chondrocytes. J Cell Biochem. May 15;92(2):316-331.
• Ionescu AM, Drissi H, Schwarz EM, Kato M, Puzas JE, McCance DJ, Rosier RN, Zuscik MJ, and O’Keefe RJ. 2004. CREB Cooperates with BMP-stimulated Smad signaling to enhance transcription of the Smad6 promoter. J Cell Physiol. Mar;198(3):428-440. Bukata SV, Gelinas J, Wei X, Rosier RN, Puzas JE, Zhang X, Schwarz EM, Song XY, Griswold DE, and O’Keefe RJ. 2004. PGE2 and IL-6 production by fibroblasts in response to titanium wear debris particles is mediated through a Cox-2 dependent pathway. J Orthop Res. Jan;22(1):6-12.